High resolution isoelectric focusing of immunoprecipitated proteins under denaturing conditions. A simple analytical method applied to the study of complement component polymorphisms.
J Immunol Methods
; 69(2): 165-72, 1984 Apr 27.
Article
in En
| MEDLINE
| ID: mdl-6232323
ABSTRACT
A simple analytical method for the study of structural protein polymorphisms is described. It consists of the immunoprecipitation of non-radiolabeled proteins using monospecific polyclonal antibodies followed by isoelectric focusing (IEF) under completely denaturing conditions in vertical polyacrylamide slab gels. The method uses small amounts of sample (usually unfractionated plasma or serum), requires no sophisticated equipment and allows the screening of large numbers of samples with comparatively small effort. This method has been applied in the identification of 2 human complement-component polymorphisms, C4-binding protein (C4-bp) and factor H (beta 1H).
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polymorphism, Genetic
/
Precipitins
/
Complement System Proteins
/
Isoelectric Focusing
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
J Immunol Methods
Year:
1984
Type:
Article