Purification of horse eosinophil peroxidase.
Biochim Biophys Acta
; 701(2): 185-91, 1982 Feb 18.
Article
in En
| MEDLINE
| ID: mdl-7041980
ABSTRACT
Eosinophil peroxidase (donor hydrogen-peroxidase oxidoreductase, EC 1.11.1.7) was isolated in a highly purified form (415/280 nm ratio, 1.05) from horse peripheral blood eosinophil. Eosinophil peroxidase was extracted from intact eosinophils (98-100% purity) or isolated eosinophil granules with 0.05 M acetate buffer (pH 4.7)/0.18 M NaCl and purified by chromatography on Sephadex G-200 and carboxymethylcellulose. Final elution was with 0.05 M acetate buffer (pH 4.7)/ 1 M NaCl. Horse eosinophil peroxidase is a strongly basic protein with bacterial properties when combined with H2O2 and iodide, bromide or, to a lesser degree, chloride.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peroxidases
/
Eosinophils
/
Horses
Limits:
Animals
Language:
En
Journal:
Biochim Biophys Acta
Year:
1982
Type:
Article