Isolation of a cDNA for chicken liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
Biochem Biophys Res Commun
; 190(2): 397-405, 1993 Jan 29.
Article
in En
| MEDLINE
| ID: mdl-7916593
ABSTRACT
A chicken liver cDNA for 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase was isolated from a Lambda ZAP2 phage library. The chicken liver cDNA codes for a protein that has 89.1, 88.4 and 88.0% amino acid identity with the human, rat and bovine liver isoforms, respectively. The kinetic properties of the rat and chicken liver enzymes, purified to homogeneity after expression in E. coli, were different including negative cooperativity for ATP binding and inhibition by Mg2+ for the chicken liver 6-phosphofructo-2-kinase but not for the rat liver kinase. Differences in the beta-loop ATP signature sequences in the chicken and rat liver kinase domains may explain the kinetic differences and represent the major divergence in the evolution of the enzyme from birds to mammals.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA
/
Chickens
/
Phosphotransferases (Alcohol Group Acceptor)
/
Phosphoric Monoester Hydrolases
/
Isoenzymes
/
Liver
Limits:
Animals
/
Humans
Language:
En
Journal:
Biochem Biophys Res Commun
Year:
1993
Type:
Article