Translocation of NLS-BSA conjugates into nuclei of permeabilized mammalian cells can be supported by protoplast extract. An experimental system for studying plant cytosolic factors involved in nuclear import.

Broder, Y C; Stanhill, A; Zakai, N; Friedler, A; Gilon, C; Loyter, A

Broder, Y C; Stanhill, A; Zakai, N; Friedler, A; Gilon, C; Loyter, A.

Affiliation

Broder YC; Department of Biological Chemistry, Institute of Life Sciences, Hebrew University of Jerusalem, Israel.

FEBS Lett ; 412(3): 535-9, 1997 Aug 04.

Article in En | MEDLINE | ID: mdl-9276462

Subject(s)

Cell Membrane Permeability; Cell Nucleus/metabolism; Cytosol/metabolism; Nuclear Proteins/pharmacokinetics; Plant Proteins/pharmacokinetics; Protoplasts/metabolism; Serum Albumin, Bovine/pharmacokinetics; Biological Transport; Cytosol/physiology; HeLa Cells; Humans; Nuclear Localization Signals; Plant Proteins/physiology

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Collection: 01-internacional Database: MEDLINE Main subject: Plant Proteins / Protoplasts / Serum Albumin, Bovine / Nuclear Proteins / Cell Membrane Permeability / Cell Nucleus / Cytosol Limits: Humans Language: En Journal: FEBS Lett Year: 1997 Type: Article Affiliation country: Israel

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