Production of a single chain anti-CEA antibody from the hybridoma cell line T84.66 using a modified colony-lift selection procedure to detect antigen-positive ScFv bacterial clones.

Rodenburg, C M; Mernaugh, R; Bilbao, G; Khazaeli, M B

Rodenburg, C M; Mernaugh, R; Bilbao, G; Khazaeli, M B.

Affiliation

Rodenburg CM; Department of Medicine, University of Alabama at Birmingham 35294-3300, USA.

Hybridoma ; 17(1): 1-8, 1998 Feb.

Article in En | MEDLINE | ID: mdl-9523232

Subject(s)

Antibodies, Neoplasm/biosynthesis; Carcinoembryonic Antigen/immunology; Immunoglobulin Fragments/biosynthesis; Immunoglobulin Variable Region/biosynthesis; Antibodies, Neoplasm/genetics; Cloning, Molecular/methods; Escherichia coli/genetics; Hybridomas; Immunoglobulin Fragments/genetics; Immunoglobulin Variable Region/genetics; Recombinant Proteins/biosynthesis; Recombinant Proteins/immunology; Selection, Genetic

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Collection: 01-internacional Database: MEDLINE Main subject: Immunoglobulin Variable Region / Immunoglobulin Fragments / Carcinoembryonic Antigen / Antibodies, Neoplasm Type of study: Prognostic_studies Language: En Journal: Hybridoma Year: 1998 Type: Article Affiliation country: United States

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