A modified method by differential adhesion for enrichment of bladder cancer stem cells
Int. braz. j. urol
; 42(4): 817-824, July-Aug. 2016. tab, graf
Article
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| LILACS
| ID: lil-794669
Biblioteca responsable:
BR1.1
ABSTRACT
ABSTRACT Purpose:
In a previous study the vaccine was effective against bladder cancer in a mouse model. However, a small portion of tumors regrew because the vaccine could not eliminate bladder cancer stem cells (CSCs). In this study, we showed a modified method for the isolation of bladder CSCs using a combination of differential adhesion method and serum-free culture medium (SFM) method. Materials andMethods:
Trypsin-resistant cells and trypsin-sensitive cells were isolated from MB49, EJ and 5637 cells by a combination of differential adhesion method and SFM method. The CSCs characterizations of trypsin-resistant cells were verified by the flow cytometry, the western blotting, the quantitative polymerase chain reaction, the resistance to chemotherapy assay, the transwell assay, and the tumor xenograft formation assay.Results:
Trypsin-resistant cells were isolated and identified in CSCs characters, with high expression of CSCs markers, higher resistance to chemotherapy, greater migration in vitro, and stronger tumorigenicity in vivo.Conclusion:
Trypsin-resistant cells displayed specific CSCs properties. Our study showed trypsin-resistant cells were isolated successfully with a modified method using a combination of differential adhesion method and SFM method.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
LILACS
Asunto principal:
Células Madre Neoplásicas
/
Neoplasias de la Vejiga Urinaria
/
Tripsina
/
Adhesión Celular
/
Separación Celular
/
Técnicas de Cultivo de Célula
Límite:
Animals
Idioma:
En
Revista:
Int. braz. j. urol
Asunto de la revista:
UROLOGIA
Año:
2016
Tipo del documento:
Article
País de afiliación:
China