Cyclooxygenase-2 expression is up-regulated in human pancreatic cancer.
Cancer Res
; 59(5): 987-90, 1999 Mar 01.
Article
en En
| MEDLINE
| ID: mdl-10070951
ABSTRACT
A large body of evidence suggests that cyclooxygenase-2 (COX-2) is important in gastrointestinal cancer. The purpose of this study was to determine whether COX-2 was expressed in adenocarcinoma of the human pancreas. Quantitative reverse transcription-PCR, immunoblotting, and immunohistochemistry were used to assess the expression of COX-2 in pancreatic tissue. Levels of COX-2 mRNA were increased by >60-fold in pancreatic cancer compared to adjacent nontumorous tissue. COX-2 protein was present in 9 of 10 cases of adenocarcinoma of the pancreas but was undetectable in nontumorous pancreatic tissue. Immunohistochemical analysis showed that COX-2 was expressed in malignant epithelial cells. In cultured human pancreatic cancer cells, levels of COX-2 mRNA and protein were induced by treatment with tumor-promoting phorbol esters. Taken together, these results suggest that COX-2 may be a target for the prevention or treatment of pancreatic cancer.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Neoplasias Pancreáticas
/
Transcripción Genética
/
Adenocarcinoma
/
Regulación Neoplásica de la Expresión Génica
/
Prostaglandina-Endoperóxido Sintasas
/
Isoenzimas
Límite:
Humans
Idioma:
En
Revista:
Cancer Res
Año:
1999
Tipo del documento:
Article
País de afiliación:
Estados Unidos