A Uve1p-mediated mismatch repair pathway in Schizosaccharomyces pombe.
Mol Cell Biol
; 19(7): 4703-10, 1999 Jul.
Article
en En
| MEDLINE
| ID: mdl-10373519
ABSTRACT
UV damage endonuclease (Uve1p) from Schizosaccharomyces pombe was initially described as a DNA repair enzyme specific for the repair of UV light-induced photoproducts and proposed as the initial step in an alternative excision repair pathway. Here we present biochemical and genetic evidence demonstrating that Uve1p is also a mismatch repair endonuclease which recognizes and cleaves DNA 5' to the mispaired base in a strand-specific manner. The biochemical properties of the Uve1p-mediated mismatch endonuclease activity are similar to those of the Uve1p-mediated UV photoproduct endonuclease. Mutants lacking Uve1p display a spontaneous mutator phenotype, further confirming the notion that Uve1p plays a role in mismatch repair. These results suggest that Uve1p has a surprisingly broad substrate specificity and may function as a general type of DNA repair protein with the capacity to initiate mismatch repair in certain organisms.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Schizosaccharomyces
/
ADN de Hongos
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Disparidad de Par Base
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Proteínas de Schizosaccharomyces pombe
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Reparación del ADN
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Endodesoxirribonucleasas
Idioma:
En
Revista:
Mol Cell Biol
Año:
1999
Tipo del documento:
Article
País de afiliación:
Estados Unidos