Time course tissue distribution of infectious salmon anaemia virus in experimentally infected Atlantic salmon Salmo salar.
Dis Aquat Organ
; 36(2): 107-12, 1999 May 12.
Article
en En
| MEDLINE
| ID: mdl-10399038
ABSTRACT
Atlantic salmon Salmo salar L. were injected intraperitoneally with infectious salmon anaemia virus (ISAV)-infective tissue homogenate to clarify the tissue distribution of ISAV in a time course study. Fish were sampled at 11 different intervals between 1 and 40 d post-infection (p.i.) and mid-kidney, head kidney, liver, spleen, intestine, gills, muscle and heart were tested for the presence of ISAV by reverse transcriptase polymerase chain reaction (RT-PCR). The results showed that during a disease outbreak, ISAV is present in most organs. It was possible to detect ISAV at all sampling times in at least 1 of the fish examined. However, for the first 8 d p.i. positive RT-PCR results were predominantly found in samples from the head kidney and mid-kidney. Fish giving positive samples after Day 13 p.i. were RT-PCR positive in most organs. These results indicated that between Days 8 to 13 p.i. considerable replication of the virus occurred, combined with wide tissue dissemination.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Orthomyxoviridae
/
Salmón
/
Infecciones por Orthomyxoviridae
/
Enfermedades de los Peces
/
Anemia
Límite:
Animals
Idioma:
En
Revista:
Dis Aquat Organ
Asunto de la revista:
BIOLOGIA
/
MEDICINA VETERINARIA
/
MICROBIOLOGIA
Año:
1999
Tipo del documento:
Article
País de afiliación:
Noruega