Early steps of replication of moloney murine leukemia virus in resting lymphocytes.
Virology
; 262(2): 408-15, 1999 Sep 30.
Article
en En
| MEDLINE
| ID: mdl-10502519
ABSTRACT
We explored the role of cell type in the early steps of replication of Moloney murine leukemia virus (Mo-MLV) by comparing viral entry and reverse transcription in physiologically quiescent peripheral blood B and T lymphocytes. Virus entry was identical in both cell types. In contrast to previous results, full-length viral DNA was synthesized in resting B lymphocytes, but in agreement with earlier reports, reverse transcription was abortive in resting T lymphocytes. The addition of exogenous nucleosides in the culture medium of resting T lymphocytes allowed reverse transcription to proceed in these cells, without inducing cell cycling. These data suggest that the difference in the ability of quiescent T and B lymphocytes to sustain reverse transcription of Mo-MLV can be explained by a difference in the dNTP pool sizes of these two populations of quiescent cells.
Buscar en Google
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Replicación Viral
/
Linfocitos
/
Virus de la Leucemia Murina de Moloney
Límite:
Animals
Idioma:
En
Revista:
Virology
Año:
1999
Tipo del documento:
Article
País de afiliación:
Francia