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Promyelocytic leukemia (PML) nuclear bodies are protein structures that do not accumulate RNA.
Boisvert, F M; Hendzel, M J; Bazett-Jones, D P.
Afiliación
  • Boisvert FM; Department of Cell Biology and Anatomy, Calgary, Alberta, Canada T2N 4N1.
J Cell Biol ; 148(2): 283-92, 2000 Jan 24.
Article en En | MEDLINE | ID: mdl-10648561
ABSTRACT
The promyelocytic leukemia (PML) nuclear body (also referred to as ND10, POD, and Kr body) is involved in oncogenesis and viral infection. This subnuclear domain has been reported to be rich in RNA and a site of nascent RNA synthesis, implicating its direct involvement in the regulation of gene expression. We used an analytical transmission electron microscopic method to determine the structure and composition of PML nuclear bodies and the surrounding nucleoplasm. Electron spectroscopic imaging (ESI) demonstrates that the core of the PML nuclear body is a dense, protein-based structure, 250 nm in diameter, which does not contain detectable nucleic acid. Although PML nuclear bodies contain neither chromatin nor nascent RNA, newly synthesized RNA is associated with the periphery of the PML nuclear body, and is found within the chromatin-depleted region of the nucleoplasm immediately surrounding the core of the PML nuclear body. We further show that the RNA does not accumulate in the protein core of the structure. Our results dismiss the hypothesis that the PML nuclear body is a site of transcription, but support the model in which the PML nuclear body may contribute to the formation of a favorable nuclear environment for the expression of specific genes.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: ARN Neoplásico / Leucemia Promielocítica Aguda / ARN Nuclear / Núcleo Celular / Proteínas de Neoplasias Idioma: En Revista: J Cell Biol Año: 2000 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: ARN Neoplásico / Leucemia Promielocítica Aguda / ARN Nuclear / Núcleo Celular / Proteínas de Neoplasias Idioma: En Revista: J Cell Biol Año: 2000 Tipo del documento: Article