Mildly oxidised low density lipoprotein induces platelet shape change via Rho-kinase-dependent phosphorylation of myosin light chain and moesin.
FEBS Lett
; 466(1): 70-4, 2000 Jan 21.
Article
en En
| MEDLINE
| ID: mdl-10648815
ABSTRACT
Oxidised low density lipoprotein (LDL) plays an important role in the pathogenesis of atherosclerosis. Here we demonstrate that mildly oxidised (mox) LDL engages the GTPase Rho and its effector molecule p160 Rho-kinase to induce phosphorylation of myosin light chain and of moesin leading to platelet shape change. Pretreatment of platelets with the selective Rho inhibitor C3-transferase from Clostridium botulinum or with the Rho-kinase inhibitor Y-27632 blocked mox-LDL-induced myosin light chain phosphorylation, moesin phosphorylation and shape change. Mox-LDL did not induce an increase in cytosolic Ca(2+) during shape change. We propose that Rho/Rho-kinase inhibition could be a strategy for prevention of the pathologic platelet activation during atherogenesis.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Plaquetas
/
Cadenas Ligeras de Miosina
/
Lipoproteínas LDL
/
Proteínas de Microfilamentos
Tipo de estudio:
Etiology_studies
Límite:
Humans
Idioma:
En
Revista:
FEBS Lett
Año:
2000
Tipo del documento:
Article
País de afiliación:
Alemania