The onset of germ cell migration in the mouse embryo.
Mech Dev
; 91(1-2): 61-8, 2000 Mar 01.
Article
en En
| MEDLINE
| ID: mdl-10704831
ABSTRACT
Mouse primordial germ cells (PGCs) are specified between embryonic day 6.5 (E6.5) and E7.5, when they have been visualized as an alkaline phosphatase-positive (AP+) cell population in the developing allantois. By E8.5, they are embedded in the hind-gut epithelium. Previous experiments have suggested different sites for PGCs' origin, and it is unclear how they reach the gut epithelium. We have used transgenic mice expressing GFP under a truncated Oct4 promoter to visualize living PGCs. We find GFP+/AP+ cells in the posterior end of the primitive streak as a dispersed population of cells actively migrating into the allantois, and directly into the adjacent embryonic endoderm. Time-lapse analysis shows these cells to be actively migratory from the time they exit the primitive streak.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Movimiento Celular
/
Células Germinativas
Límite:
Animals
Idioma:
En
Revista:
Mech Dev
Asunto de la revista:
EMBRIOLOGIA
Año:
2000
Tipo del documento:
Article
País de afiliación:
Estados Unidos