[Confirmation by molecular typing of cross-contamination in a mycobacteria laboratory]. / Confirmación mediante tipificación molecular de contaminaciones cruzadas en el laboratorio de micobacterias.
Enferm Infecc Microbiol Clin
; 18(1): 12-5, 2000 Jan.
Article
en Es
| MEDLINE
| ID: mdl-10721556
ABSTRACT
BACKGROUND:
Detection of cross-contamination in the laboratory by restriction fragments length polymorphism (RFLP) analysis of Mycobacterium tuberculosis strains. MATERIAL ANDMETHODS:
224 strains isolated during a five years period were characterized by IS6110 fingerprinting performed (RFLP) by standardized protocols.RESULTS:
Four groups of isolates with smear-negative specimens and low number of colony form its units were detected. They were processed in the same batch and day than other smear-positive specimens with identical RFLP patterns. Fifteen patients were involved and the review of four patients' charts showed that they did not have the typical manifestations of tuberculosis.CONCLUSIONS:
When M. tuberculosis isolates were obtained from smear-negative specimens, the results of specimens processed in the same batch and the patients' charts should be reviewed. If with these data the possibility of cross-contamination is suspected, the isolates must be analyzed by molecular typing methods.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Técnicas de Laboratorio Clínico
/
Mycobacterium tuberculosis
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
Es
Revista:
Enferm Infecc Microbiol Clin
Asunto de la revista:
DOENCAS TRANSMISSIVEIS
/
MICROBIOLOGIA
Año:
2000
Tipo del documento:
Article