Detection of Ureaplasma diversum in cattle using a newly developed PCR-based detection assay.
Vet Microbiol
; 72(3-4): 241-50, 2000 Mar 15.
Article
en En
| MEDLINE
| ID: mdl-10727834
ABSTRACT
Ureaplasma diversum has been associated with different clinical manifestations including bovine vulvitis, endometritis, salpingitis, spontaneous abortion and infertility. Because the isolation of this ureaplasma from clinical samples is difficult, there is a need for improved detection methods. We developed a PCR assay based on amplification of a region of the gene encoding 16S rRNA. The specificity of the amplification was verified by sequence analysis. Female bovine vaginal swabs (n=168) were collected and the presence of U. diversum evaluated by both culture methods and by the PCR assay. Culture was positive for 60 samples (35.7%), and PCR-specific amplification was obtained for 89 samples (52.9%). These results indicated a high prevalence of U. diversum in the selected animals and the higher sensitivity of this PCR assay as compared to culture.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Ureaplasma
/
Enfermedades de los Bovinos
/
Reacción en Cadena de la Polimerasa
/
Infecciones por Ureaplasma
Tipo de estudio:
Diagnostic_studies
/
Etiology_studies
/
Risk_factors_studies
Límite:
Animals
Idioma:
En
Revista:
Vet Microbiol
Año:
2000
Tipo del documento:
Article
País de afiliación:
Brasil