Morphine treatment affects the regulation of high mobility group I-type chromosomal phosphoproteins in C6 glioma cells.
Life Sci
; 66(18): 1725-31, 2000 Mar 24.
Article
en En
| MEDLINE
| ID: mdl-10809169
ABSTRACT
High mobility group (HMG) I-type chromosomal phosphoproteins HMG I/Y and HMG I-C were investigated following morphine treatment of C6BU-1 glioma. Cells were labelled with [32P]-orthophosphoric acid. Electrophoretic profiles and autoradiograms of the control cells revealed the presence of HMG I and HMG I-C proteins. HMG Y was not detected. Northern blot analysis showed a single HMG I/Y transcript. Treatment with morphine lowered the [32P]-incorporation in HMG I and HMGI-C proteins and the level of the HMG I/Y transcript. However, it did not change the protein ratios on the Coomassie stained gels. These results suggest that morphine may trigger independent reaction pathways affecting either transcription regulation and/or postsynthetic phosphorylation of the preexisting HMG I-type proteins. In addition, opposing changes in the postsynthetic phosphorylation of HMG 14 and histones H1AB were also noticed.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Factores de Transcripción
/
Neoplasias Encefálicas
/
Proteínas del Grupo de Alta Movilidad
/
Cromosomas
/
Glioma
/
Analgésicos Opioides
/
Morfina
Límite:
Animals
Idioma:
En
Revista:
Life Sci
Año:
2000
Tipo del documento:
Article
País de afiliación:
Bulgaria