Novel opioid peptides endomorphin-1 and endomorphin-2 are present in mammalian immune tissues.
J Neuroimmunol
; 106(1-2): 53-9, 2000 Jul 01.
Article
en En
| MEDLINE
| ID: mdl-10814782
ABSTRACT
Endomorphin (EM)-1 and EM-2 are opioid tetrapeptides, reported within the central nervous system, which have very high specificity and affinity for the mu-opioid receptor. We have used newly developed and well-characterised radioimmunoassays (RIAs) in combination with reversed-phase high-performance liquid chromatography (HPLC) to detect EM-1 and EM-2 immunoreactivity (ir) in rat immune tissues. Endomorphins were detectable in extracts of rat spleen (total EM-1-ir/spleen 440+/-73 pg, mean+/-SEM, a=group of eight rats; EM-2-ir 150+/-12 pg) and thymus (EM-1-ir 152+/-18 pg, mean+/-SEM n=8; EM-2-ir 156+/-28 pg). EM-2-ir was detectable in extracts of human spleen (338+/-196 pg/g tissue, n=3). Multiple peaks of EM-1-ir and EM-2-ir were observed in rat spleen and thymus extracts, and multiple peaks of EM-2-ir were observed in extracts of human spleen, following reversed-phase HPLC and RIAs. This is the first report of endomorphin immunoreactivity in tissues of the rat and human immune systems.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Oligopéptidos
/
Sistema Inmunológico
Límite:
Adult
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Animals
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Female
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Humans
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Male
/
Middle aged
Idioma:
En
Revista:
J Neuroimmunol
Año:
2000
Tipo del documento:
Article
País de afiliación:
Reino Unido