The salivary adenosine deaminase from the sand fly Lutzomyia longipalpis.
Exp Parasitol
; 95(1): 45-53, 2000 May.
Article
en En
| MEDLINE
| ID: mdl-10864517
ABSTRACT
In the process of sequencing a subtracted cDNA library from the salivary glands of the sand fly Lutzomyia longipalpis, we identified a cDNA with similarities to gene products of the adenosine deaminase family. Prompted by this cDNA finding, we detected adenosine deaminase activity at levels of 1 U/mg protein in salivary gland homogenates. The activity was significantly reduced following a blood meal indicating its apparent secretory fate. The native enzyme has a K(m) of approximately 10 microM, an isoelectric pH between 4.5 and 5.5, and an apparent molecular weight of 52 kDa by size exclusion chromatography. The possible role of this enzyme, which converts adenosine to inosine, in the feeding physiology of L. longipalpis is discussed.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Psychodidae
/
Adenosina Desaminasa
/
ADN Complementario
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Exp Parasitol
Año:
2000
Tipo del documento:
Article
País de afiliación:
Estados Unidos