Plasma membrane coating with cationic silica particles and osmotic shock alters the morphology of bovine aortic endothelial cells.
Biochim Biophys Acta
; 1467(1): 85-90, 2000 Jul 31.
Article
en En
| MEDLINE
| ID: mdl-10930511
ABSTRACT
We have used a published method of membrane preparation based on the precoating of the apical membrane of aortic endothelial cells with cationic silica microbeads (with or without polyacrylic acid) in combination with an osmotic shock and mechanical shearing to isolate the apical from the basal plasma membranes of these cells, in vitro. After labeling of the plasma membrane of adherent endothelial cells with a fluorescent derivative of phosphatidylcholine and by using laser confocal fluorescence scanning microscopy, we found that this method of membrane isolation rapidly induced invaginations of the basal plasma membrane to an extent which makes this method unsuitable for further membrane lipid analysis. Morphological analysis of the cells and fluorescence recovery after photobleaching experiments on the plasma membranes were performed at each step of the purification procedure and showed that only hypotonic shock and mechanical shearing of the cells enabled the basal plasma membranes to be purified without significant morphological changes.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Endotelio Vascular
/
Membrana Celular
/
Dióxido de Silicio
Límite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
2000
Tipo del documento:
Article
País de afiliación:
Francia