Gamma-glutamyltranspeptidase-dependent glutathione catabolism results in activation of NF-kB.
Biochem Biophys Res Commun
; 276(3): 1062-7, 2000 Oct 05.
Article
en En
| MEDLINE
| ID: mdl-11027590
ABSTRACT
gamma-glutamyltranspeptidase (GGT) is a key enzyme implicated in the homeostasis of intracellular reduced glutathione (GSH) and hence in the regulation of the cellular redox state. Besides, the extracellular cleavage of GSH by GGT leads to reactive oxygen species (ROS) production, depending on the generation and enhanced reactivity of cysteinylglycine (CysGly). Using a model cell line, the V79 GGT, which highly expresses a human GGT transgene, we examined whether the GGT induced oxidant stress could modulate intracellular transcription factors. For the first time, we show that GGT-dependent ROS production induces the NF-kB-binding and transactivation activities. This induction mimicked the one observed by H(2)O(2) and was inhibited by catalase, suggesting the involvement of H(2)O(2) in the NF-kB activation.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
FN-kappa B
/
Gamma-Glutamiltransferasa
/
Glutatión
Tipo de estudio:
Prognostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2000
Tipo del documento:
Article
País de afiliación:
Francia