Differences in DNA-sequence recognition between the DNA-binding domain fragment and the full-length molecule of the heat-shock transcription factor of schistosome.
Biochim Biophys Acta
; 1519(3): 230-4, 2001 Jun 28.
Article
en En
| MEDLINE
| ID: mdl-11418190
ABSTRACT
Binding and inhibition studies reveal that the DNA-binding domain (DBD) fragment and the full-length molecule of the heat-shock transcription factor of schistosome (SmHSF) differ in DNA sequence recognition. SmHSF does not recognize the ideal HSE consensus sequence (nGAAnnTTCnnGAAn) but recognizes a variant HSE that contains nGTAn instead of nGAAn in the third pentamer. The DBD reacts efficiently with the ideal HSE sequence and with lower affinity with the variant HSE sequence. These findings suggest that elements inside and outside the DBD contribute to the DNA-binding specificity of HSF.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Schistosoma mansoni
/
Proteínas de Unión al ADN
/
Proteínas de Choque Térmico
Límite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
2001
Tipo del documento:
Article
País de afiliación:
Israel