Analysis of glutathione by capillary electrophoresis based on sample stacking.
Electrophoresis
; 22(11): 2351-3, 2001 Jul.
Article
en En
| MEDLINE
| ID: mdl-11504072
ABSTRACT
Glutathione is a small peptide, which participates in cellular oxidation-reduction and detoxification. It is present in most biological tissues at different concentrations. The oxidized and reduced forms of the peptide were measured in erythrocytes and myocardial tissue by capillary electrophoresis based on stacking. After tissue homogenization or hemolysis of the red blood cells, the samples were deproteinized with acetonitrile and injected filling about 13% of the capillary volume. The electrophoresis was performed at 10 kV using a separation buffer of 250 mM borate, 50 mM Tris, pH 8.0. Sample stacking increased the sensitivity of detection by 10-20-fold.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Electroforesis Capilar
/
Glutatión
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
Electrophoresis
Año:
2001
Tipo del documento:
Article
País de afiliación:
Estados Unidos