A1 adenosine receptor activation inhibits neurite process formation by Rho kinase-mediated pathways.
Neuroreport
; 12(14): 3057-63, 2001 Oct 08.
Article
en En
| MEDLINE
| ID: mdl-11568636
ABSTRACT
A1 adenosine receptors (A1ARs) are expressed in the brain during critical periods of neurogenesis and neuronal differentiation. To examine influences of A1AR activation on neuronal development we studied the effects of A1AR activation on process growth in PC12 cells expressing A1ARs and in primary cultures of cortical and hippocampal neurons. In PC12 cells, we found that A1AR activation potently inhibited nerve growth factor (NGF)-induced neurite growth and induced stress fiber formation. A1ARs action was not mediated by inhibition of p44/42 MAP kinase activity, as inhibition of MEK/MAP kinase had no effects on A1AR action. When Rho kinase activity was blocked, A1AR agonists no longer inhibited neurite growth and stress fiber formation was blocked. In neurons, A1AR activation also inhibited process growth, and A1AR action was also mediated by Rho kinase. These data show that A1AR activation inhibits neurite growth and that the inhibitory effects of A1AR are dependent on Rho kinase.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Encéfalo
/
Transducción de Señal
/
Diferenciación Celular
/
Neuritas
/
Proteínas Serina-Treonina Quinasas
/
Receptores Purinérgicos P1
Límite:
Animals
Idioma:
En
Revista:
Neuroreport
Asunto de la revista:
NEUROLOGIA
Año:
2001
Tipo del documento:
Article
País de afiliación:
Estados Unidos