HAT activity is essential for CBP-1-dependent transcription and differentiation in Caenorhabditis elegans.
EMBO Rep
; 3(1): 50-5, 2002 Jan.
Article
en En
| MEDLINE
| ID: mdl-11751575
ABSTRACT
The p300/CBP family of transcriptional coactivators possesses multiple functional domains, including a histone acetyltransferase (HAT) and several activation domains. A number of models have been proposed to account for their roles in transcriptional activation, including interactions with basal transcription machinery and chromatin remodeling. However, individual contributions of these domains to transcriptional activation and their significance in living organisms remain unclear. We addressed the importance of the HAT activity of CBP-1, the worm ortholog of p300/CBP, in Caenorhabditis elegans with three different and complementary approaches. These include allele-specific RNA-mediated interference (RNAi), genetic rescue and the use of a specific chemical inhibitor of the p300/CBP HAT. Our findings demonstrate that HAT activity is of primary importance for CBP-1 to regulate transcription and to promote differentiation during C. elegans embryogenesis.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Acetiltransferasas
/
Factores de Transcripción
/
Transcripción Genética
/
Proteínas del Helminto
/
Diferenciación Celular
/
Caenorhabditis elegans
/
Proteínas de Saccharomyces cerevisiae
/
Proteínas de Caenorhabditis elegans
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
EMBO Rep
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2002
Tipo del documento:
Article
País de afiliación:
Estados Unidos