The effect of mechanical force on mRNA levels of collagenase, collagen type I, and tissue inhibitors of metalloproteinases in gingivae of dogs.
J Dent Res
; 80(12): 2080-4, 2001 Dec.
Article
en En
| MEDLINE
| ID: mdl-11808766
ABSTRACT
Orthodontic force causes an injury to and subsequent degradation of the attachment apparatus, thus leading to the transposition of the tooth. The gingiva, however, is compressed and sometimes becomes hypertrophic with tooth movement and often shrinks after treatment. To study the effect of force on the gingiva, we applied orthodontic force in dogs and analyzed gingival tissues 1, 2, 3, 7, 14, and 28 days later as well as after removing the force. The effect of force on mRNA levels of collagen type I (col-I), matrix metalloproteinase-1 (MMP- 1), and tissue inhibitors 1 and 2 (TIMPs) genes was analyzed by RT-PCR, and MMP-1 activity was determined by zymography. The results showed that force significantly increased both the mRNA levels of MMP-1 and its interstitial activity. After the removal of force, MMP-1 gene expression was significantly decreased. The results could partly explain the clinically observed shrinkage and adaptation of the gingiva during tooth movement.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Técnicas de Movimiento Dental
/
Colagenasas
/
Inhibidores Tisulares de Metaloproteinasas
/
Metaloproteinasa 1 de la Matriz
/
Colágeno Tipo I
Límite:
Animals
Idioma:
En
Revista:
J Dent Res
Año:
2001
Tipo del documento:
Article
País de afiliación:
Israel