Deregulated DNA polymerase beta strengthens ionizing radiation-induced nucleotidic and chromosomal instabilities.
Oncogene
; 21(15): 2320-7, 2002 Apr 04.
Article
en En
| MEDLINE
| ID: mdl-11948415
ABSTRACT
DNA polymerase beta (Pol beta) is an error-prone enzyme which has been found to be overexpressed in several human tumors. By using a couple of recombinant CHO cells differing only from the exogenous expression of Pol beta, we showed here that cells overexpressing Pol beta are much more sensitive to IR treatments by increasing apoptosis. We also found that the surviving cells displayed an hypermutator phenotype which could be explained by different pathways involving Pol beta, such as (i) an increased capacity to incorporate into DNA the mutagenic dGTP analog, 8-oxo-dGTP, one of the most abundant purine-derived nucleotides exposed to gamma-irradiation, (ii) the induction of IR-induced DNA breaks and (iii) accumulation of chromosome aberrations induced by radiation. Alteration of Pol beta expression in irradiated cells thus appears to strengthen both cell death and genetic changes associated with a malignant phenotype. These data provide new insights into the cellular response to radiations and the associated carcinogenic consequences.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Radiación Ionizante
/
Daño del ADN
/
Aberraciones Cromosómicas
/
ADN Polimerasa beta
Límite:
Animals
Idioma:
En
Revista:
Oncogene
Asunto de la revista:
BIOLOGIA MOLECULAR
/
NEOPLASIAS
Año:
2002
Tipo del documento:
Article
País de afiliación:
Francia