Rat nigral xenografts survive in the brain of MHC class II-, but not class I-deficient mice.
Neuroscience
; 115(2): 495-504, 2002.
Article
en En
| MEDLINE
| ID: mdl-12421616
ABSTRACT
We have examined the role of the indirect pathway of antigen recognition and T cells in neural xenografts rejection by using major histocompatibility complex (MHC) class II-deficient mice as xenograft recipients. Dissociated embryonic ventral mesencephalic tissue from Sprague-Dawley rats was stereotaxically injected as a cell suspension into the striatum of MHC class II-deficient adult mice as well as MHC class I-deficient and wild-type mice as controls. All of the MHC class II-deficient mice had surviving grafts in the striatum 4 weeks post-grafting. In contrast, only a few of the MHC class I-deficient mice exhibited very small grafts and none of the wild-type mice had any surviving grafts. The mean number of surviving transplanted dopamine neurons in the MHC class II-deficient group was significantly larger than that observed in the other two groups. Moderate levels of MHC class I antigen expression were seen in the transplantation sites of some animals in the MHC class II-deficient group. No helper or cytotoxic T cells were observed infiltrating into the graft sites of this group. However, there were markedly increased levels of expression of MHC class I and class II antigens, and a number of T cells infiltrating in the graft sites in both the MHC class I-deficient and wild-type groups. These results show that rat embryonic nigral tissue can survive transplantation in the brain of the MHC class II-deficient mice for at least 4 weeks without any overt signs of rejection, suggesting that the indirect pathway of foreign antigen recognition mediated by host MHC class II molecules and helper T cells plays an important role in the rejection responses to intracerebral xenografts.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Sustancia Negra
/
Antígenos de Histocompatibilidad Clase I
/
Antígenos de Histocompatibilidad Clase II
/
Supervivencia de Injerto
Límite:
Animals
Idioma:
En
Revista:
Neuroscience
Año:
2002
Tipo del documento:
Article
País de afiliación:
Estados Unidos