The effect of ribose 5-phosphate and 5-phosphoribosyl-1-pyrophosphate availability on de novo synthesis of purine nucleotides in rat liver slices.
Biochim Biophys Acta
; 432(1): 10-7, 1976 Apr 15.
Article
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| MEDLINE
| ID: mdl-1260047
ABSTRACT
The effect of increasing cellular ribose 5-phosphate (ribose-5-P) availability by methylene blue-induced acceleration of the oxidative pentose phosphate pathway on the rate of 5-phosphoribosyl-1-pyrophosphate (P-ribose-PP) generation, was studied in slices of rat liver at varying Pi concentration. It was found that at Pi concentration prevailing in the tissue of extracellular physiological Pi concentration, ribose-5-P availability is saturating for P-ribose-PP generation, as gauged by the rate of adenine incorporation into tissue nucleotides. The effect of altering P-ribose-PP availability on the rate of de novo purine production gauged by the rate of formate incorporation into purines, was also studied. It was found that the physiological P-ribose-PP concentration in rat liver tissue is limiting for purine synthesis de novo. Depletion of cellular P-ribose-PP, achieved by increase of P-ribose-PP consumption, decelerated purine synthesis, while increase of P-ribose-PP availability, achieved by activation of P-ribose-PP synthetase occurring at elevated Pi concentration, resulted in acceleration of purine synthesis.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Pentosafosfatos
/
Fosforribosil Pirofosfato
/
Nucleótidos de Purina
/
Ribosamonofosfatos
Límite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
1976
Tipo del documento:
Article