Netrin-1/neogenin interaction stabilizes multipotent progenitor cap cells during mammary gland morphogenesis.
Dev Cell
; 4(3): 371-82, 2003 Mar.
Article
en En
| MEDLINE
| ID: mdl-12636918
ABSTRACT
Netrin-1 and its receptors play an essential role patterning the nervous system by guiding neurons and axons to their targets. To explore whether netrin-1 organizes nonneural tissues, we examined its role in mammary gland morphogenesis. Netrin-1 is expressed in prelumenal cells, and its receptor neogenin is expressed in a complementary pattern in adjacent cap cells of terminal end buds (TEBs). We discovered that loss of either gene results in disorganized TEBs characterized by exaggerated subcapsular spaces, breaks in basal lamina, dissociated cap cells, and an increased influx of cap cells into the prelumenal compartment. Cell aggregation assays demonstrate that neogenin mediates netrin-1-dependent cell clustering. Thus, netrin-1 appears to act locally through neogenin to stabilize the multipotent progenitor (cap) cell layer during mammary gland development. Our results suggest that netrin-1 and its receptor neogenin provide an adhesive, rather than a guidance, function during nonneural organogenesis.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Células Madre
/
Adhesión Celular
/
Diferenciación Celular
/
Células Epiteliales
/
Glándulas Mamarias Animales
/
Proteínas de la Membrana
/
Factores de Crecimiento Nervioso
Límite:
Animals
Idioma:
En
Revista:
Dev Cell
Asunto de la revista:
EMBRIOLOGIA
Año:
2003
Tipo del documento:
Article
País de afiliación:
Estados Unidos