Determination of efficiency of attachment of biotinylated antibodies to avidin-linked, aqueous-based microcapsules by flow cytometry.

Chow, Andrea A; Moser, Charlotte A; Speaker, Tully J; Offit, Paul A

Chow, Andrea A; Moser, Charlotte A; Speaker, Tully J; Offit, Paul A.

Afiliación

Chow AA; University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.

J Immunol Methods ; 277(1-2): 135-9, 2003 Jun 01.

Article en En | MEDLINE | ID: mdl-12799046

ABSTRACT

ABSTRACT

We used flow cytometry to determine the percentage of aqueous-based microcapsules bearing antibodies specific for various antigen-presenting cells (APCs) within a given population of putative APC-specific microcapsules. Flow cytometry offers a high-throughput, rapid and simple method to analyze antibody binding to noncellular, nonspherical material.

Asunto(s)

Anticuerpos/metabolismo; Células Presentadoras de Antígenos/metabolismo; Avidina/metabolismo; Citometría de Flujo/métodos; Animales; Biotina/metabolismo; Cápsulas

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Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Avidina / Citometría de Flujo / Anticuerpos / Células Presentadoras de Antígenos Límite: Animals Idioma: En Revista: J Immunol Methods Año: 2003 Tipo del documento: Article País de afiliación: Estados Unidos

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