Simultaneous amplification and detection of specific DNA sequences.
Biotechnology (N Y)
; 10(4): 413-7, 1992 Apr.
Article
en En
| MEDLINE
| ID: mdl-1368485
ABSTRACT
We have enhanced the polymerase chain reaction (PCR) such that specific DNA sequences can be detected without opening the reaction tube. This enhancement requires the addition of ethidium bromide (EtBr) to a PCR. Since the fluorescence of EtBr increases in the presence of double-stranded (ds) DNA an increase in fluorescence in such a PCR indicates a positive amplification, which can be easily monitored externally. In fact, amplification can be continuously monitored in order to follow its progress. The ability to simultaneously amplify specific DNA sequences and detect the product of the amplification both simplifies and improves PCR and may facilitate its automation and more widespread use in the clinic or in other situations requiring high sample throughput.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
ADN
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Biotechnology (N Y)
Año:
1992
Tipo del documento:
Article