Patterns of prohormone processing. Order revealed by a new procholecystokinin-derived peptide.
J Biol Chem
; 267(3): 1517-21, 1992 Jan 25.
Article
en En
| MEDLINE
| ID: mdl-1370477
ABSTRACT
An 83-amino acid cholecystokinin peptide with a sulfated tyrosine and an amidated carboxyl terminus (CCK-83) was purified from human intestinal mucosa. The purified peptide was chemically characterized, and its bioactivity was compared to CCK-8. Several post-translational processing steps such as cleavage at basic residues, sulfation, and amidation are necessary to form biologically active cholecystokinin from its nascent prepropeptide. The discovery of CCK-83 gives new insight into the order of preprohormone processing. The processing of prepro-CCK appears to be in the order of 1) signal peptidase cleavage, 2) tyrosine sulfation, 3) cleavage after a carboxyl-terminal pair of basic residues, 4) carboxypeptidase B-like cleavage of these basic residues, 5) amidation (which results in the formation of CCK-83), and 6) cleavage at monobasic residues by endopeptidases (which results in the smaller molecular forms of cholecystokinin). The characterization of biologically active CCK-83 with a sulfated tyrosine and an amidated carboxyl terminus establishes the site of signal peptidase action and suggests an order of post-translational modifications that give rise to the various molecular forms of cholecystokinin.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Endopeptidasas
/
Precursores de Proteínas
/
Colecistoquinina
/
Serina Endopeptidasas
/
Procesamiento Proteico-Postraduccional
/
Mucosa Intestinal
/
Proteínas de la Membrana
Límite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
J Biol Chem
Año:
1992
Tipo del documento:
Article