Balbiani ring hnRNP substructure visualized by selective staining and electron spectroscopic imaging.
J Cell Biol
; 117(3): 483-91, 1992 May.
Article
en En
| MEDLINE
| ID: mdl-1374066
ABSTRACT
The Balbiani Rings (BR) in the polytene chromosomes of Chironomus salivary glands are intense sites of transcription. The nascent RNPs fold during transcription into 40-50-nm granules, containing in the mature transcript approximately 37-kb RNA. Using a new nucleic acid specific stain, osmium ammine B on Lowicryl sections, in combination with electron energy filtered imaging of sections containing BR granules, we demonstrate a RNA-rich particulate substructure (10-nm particle diameter; 10-12 particles per BR granule). Elemental imaging supports that these particles are enriched in phosphorus. The possible relationship of these RNA-rich particles to ribonucleosomes is discussed, as well as models for their arrangement in the mature BR granules.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Ribonucleoproteínas
/
Glándulas Salivales
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Chironomidae
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Cromosomas
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Compuestos de Osmio
Límite:
Animals
Idioma:
En
Revista:
J Cell Biol
Año:
1992
Tipo del documento:
Article