Differential pp60c-src activity in well and poorly differentiated human colon carcinomas and cell lines.
J Clin Invest
; 90(3): 815-21, 1992 Sep.
Article
en En
| MEDLINE
| ID: mdl-1381724
ABSTRACT
The results presented in this report demonstrate increased pp60c-src kinase activity associated with moderate to well differentiated colon tumors, corroborating previous observations by other groups. Extension of this analysis to include a small number of poorly differentiated colon carcinomas revealed src kinase activity comparable to that observed in normal colonic mucosa, considerably less than that observed in moderate/well differentiated lesions. Correlations of src kinase activity with differentiation was confirmed within a panel of colon cell lines where increased activity, associated with moderate/well differentiated lines, was accompanied by increased expression of pp60c-src protein. Use of an antiphosphotyrosine antibody in immunoprecipitation revealed the presence of novel phosphotyrosyl cellular substrates in human colon cell lines displaying elevated pp60c-src kinase activity. These observations suggest a role for the src protooncogene in colonic differentiation pathways.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Proto-Oncogénicas pp60(c-src)
/
Neoplasias del Colon
Límite:
Humans
Idioma:
En
Revista:
J Clin Invest
Año:
1992
Tipo del documento:
Article