A gas chromatographic procedure for separation and quantitation of the enantiomers of the antidepressant tranylcypromine.
Biochem Pharmacol
; 44(9): 1894-7, 1992 Nov 03.
Article
en En
| MEDLINE
| ID: mdl-1449543
ABSTRACT
A novel assay procedure has been developed that allows for the separation and quantification of the enantiomers of the monoamine oxidase inhibitor tranylcypromine (TCP) in brain and liver of rats. The analytical method involves extraction of the drug from rat tissue with an organic solvent. TCP is then derivatized with S-(-)-N-(trifluoroacetyl)-prolyl chloride to allow gas chromatographic analysis of the resulting diastereoisomers. Conditions for analysis by a gas chromatograph equipped with a nitrogen-phosphorus detector and a capillary column are described. The method has been applied to the separation and quantification of the enantiomers of TCP in samples of brain and liver of rats that had been injected with this drug alone and after pretreatment with iprindole, a drug known to block aromatic ring hydroxylation.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Tranilcipromina
Límite:
Animals
Idioma:
En
Revista:
Biochem Pharmacol
Año:
1992
Tipo del documento:
Article
País de afiliación:
Canadá