Nucleolar segregation lags behind the rest of the genome and requires Cdc14p activation by the FEAR network.
Cell Cycle
; 3(4): 496-502, 2004 Apr.
Article
en En
| MEDLINE
| ID: mdl-15004526
ABSTRACT
In order to transmit a full genetic complement cells must ensure that all chromosomes are accurately split and distributed during anaphase. Chromosome XII in S. cerevisiae contains the site of nucleolar assembly, a 1-2Mb array of rDNA genes named RDN1. Cdc14p is a conserved phosphatase, essential for anaphase progression and mitotic exit, which is kept inactive at the nucleolus until mitosis. In early anaphase, the FEAR network (Cdc Fourteen Early Anaphase Release) promotes the transient and partial release of Cdc14p from the nucleolus. The putative role of Cdc14p released by the FEAR network is thought to be the stimulation of full Cdc14p release by activation of the GTPase-driven signaling cascade (the Mitotic Exit Network or MEN) that ensures mitotic exit. Here, we show that nucleolar segregation is spatially separated and temporally delayed from the rest of the genome. Nucleolar segregation occurs during mid-anaphase and coincides with the FEAR release of Cdc14p. Inactivation of FEAR delays nucleolar segregation until late anaphase, demonstrating that one function of the FEAR network is to promote segregation of repetitive nucleolar chromatin during mid-anaphase.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Nucléolo Celular
/
Proteínas Tirosina Fosfatasas
/
Genoma
/
Proteínas de Ciclo Celular
/
Proteínas de Saccharomyces cerevisiae
Límite:
Humans
Idioma:
En
Revista:
Cell Cycle
Año:
2004
Tipo del documento:
Article
País de afiliación:
Reino Unido