The stoichiometry of the four linker subunits of Lumbricus terrestris hemoglobin suggests an asymmetric distribution.
Micron
; 35(1-2): 127-9, 2004.
Article
en En
| MEDLINE
| ID: mdl-15036315
ABSTRACT
The extracellular, giant ( approximately 3.6 MDa) hexagonal bilayer hemoglobin of the earthworm Lumbricus terrestris consists of 12 dodecamers of globin chains tethered to a central complex of 36 non-globin, linker chains (24-32 kDa). Four types of linker chains L1-L4 have been detected by electrospray ionization (ESI) and by matrix-assisted laser desorption ionization (MALDI) mass spectrometry (MS) and isolated by reversed phase high pressure liquid chromatography (HPLC). Deconvolution of the HPLC elution profile and of the MS spectra provided the following individual linker contents, expressed as percent of the sum of the four linker peak areas HPLC-21% L1, 37% L2, 23% L3 and 19% L4, MALDI-47% L1, 29% L2, 16% L3 and 8% L4; ESI-24% L1, 16% L2, 40% L3 and 20% L4; respectively. Comparison with electrophoretic results revealed a surprising lack of overall agreement between all the methods. The calculated mean values of the available linker contents were found to be 32+/-12% L1, 28+/-9% L2, 27+/-10% L3 and 13+/-7% L4, suggesting the following relative stoichiometry L1 L2 L3 L4 approximately 1 1 1 0.5. With a total of 36 linkers, a hexagonally symmetric distribution of each of the four linker chains is impossible. Thus, the asymmetric linker distribution provides an explanation for the existence of a large dipole moment of Lumbricus terrestris hemoglobin, 17,300+/-2300 Da (Takashima et al., 1999).
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Oligoquetos
/
Hemoglobinas
Límite:
Animals
Idioma:
En
Revista:
Micron
Asunto de la revista:
DIAGNOSTICO POR IMAGEM
Año:
2004
Tipo del documento:
Article
País de afiliación:
Estados Unidos