[Characterization of human lung cancer cell line A549 transfected with human interferon-gamma gene].
Zhejiang Da Xue Xue Bao Yi Xue Ban
; 33(2): 125-8, 2004 03.
Article
en Zh
| MEDLINE
| ID: mdl-15067732
ABSTRACT
OBJECTIVE:
To establish a human lung cancer cell line expressing human interferon-gamma.METHODS:
The full-length gene of human interferon-gamma (IFN-gamma) was introduced into the human lung adenocarcinoma cell line A549 through retroviral vector pLXSN. The established cell line A549-IFN-gamma was tested for expression of MHC class I and class II by flow cytometer (FCM) and tested for expression of IFN-gamma by enzyme-lined immunoadsorbent assay (ELISA ). The tumorigenesis of cell line A549-IFN-gamma was tested on nude mice.RESULTS:
A high level of IFN-gamma protein was detected in the culture supernatants of cell line A549-IFN-gamma. The expressions of MHC class I and class II on A549-IFN-gamma cells increased significantly (P<0.01), when compared with parental cell line A549. However, there was no significant difference (P<0.05) between the growth of cell line A549-IFN-gamma and A549. Finally, the tumorigenesis test showed that A549-IFN-gamma had lower tumorigenetic effects than A549.CONCLUSION:
The results indicate that introduction of human IFN-gamma gene into cell line A549 could increase its immunogenicity and decrease its tumorigenesis. With the established cell line A549-IFN-gamma, a tumor vaccine for human lung cancer may be developed.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Terapia Genética
/
Interferón gamma
/
Neoplasias Pulmonares
Límite:
Animals
/
Humans
/
Male
Idioma:
Zh
Revista:
Zhejiang Da Xue Xue Bao Yi Xue Ban
Asunto de la revista:
MEDICINA
Año:
2004
Tipo del documento:
Article
País de afiliación:
China