Identification of genes induced in wound-treated wild rice (Oryza minuta).
Mol Cells
; 17(2): 230-6, 2004 Apr 30.
Article
en En
| MEDLINE
| ID: mdl-15179035
ABSTRACT
A subtracted library was constructed from wound-treated wild rice (Oryza minuta) by suppression subtractive hybridization (SSH) in combination with mirror orientation selection (MOS). To distinguish between differentially expressed transcripts and false positive clones, DNA chips containing 960 random clones were applied as a form of reverse Northern screening. Based on the signal intensities and expression ratios obtained from experiments performed in triplicate, 371 clones were selected. ESTs produced from the subtracted library showed 63.2% redundancy, and 72% of all clones could be matched to the GenBank nonredundant database. Functional categorization placed the identified enriched genes in categories of subcellular localization, metabolism, cell rescue and defense, and transcription. These EST-related resistance mechanisms could be used in investigations into the defense mechanisms of wild species, and to provide new routes to improving the germplasm of cultivated rice.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Oryza
/
Etiquetas de Secuencia Expresada
/
Perfilación de la Expresión Génica
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Mol Cells
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2004
Tipo del documento:
Article