Glutathione content and adaptation to endogenously induced energy depletion in Mv1Lu cells.
Free Radic Biol Med
; 36(12): 1555-65, 2004 Jun 15.
Article
en En
| MEDLINE
| ID: mdl-15182857
ABSTRACT
Transfection of genes that code for enzymes of energy metabolism provides alternative models to study the adaptive response to energy restriction induced by endogenous changes instead of by unfavorable environmental conditions. Overexpression of the glycolytic enzyme fructose-2,6-bisphosphatase reduced the content of fructose 2,6-bisphosphate, inducing energy limitation in the mink lung epithelial cell line Mv1Lu. This metabolic stress reduced the ATP available in transfected cells by 20%, which downregulated active ion transport and protein turnover. Ion homeostasis and cell function require concomitant reductions in cell membrane ion permeability and protein damage. Our results indicate that glutathione content linked these features of the adaptive response to the endogenously induced metabolic downregulation.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Glutatión
Límite:
Animals
Idioma:
En
Revista:
Free Radic Biol Med
Asunto de la revista:
BIOQUIMICA
/
MEDICINA
Año:
2004
Tipo del documento:
Article
País de afiliación:
España