Synthesis of long-chain polyunsaturated fatty acids is inhibited in vivo in hypercholesterolemic rabbits and in vitro by oxysterols.
Prostaglandins Leukot Essent Fatty Acids
; 71(2): 79-86, 2004 Aug.
Article
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| MEDLINE
| ID: mdl-15207522
ABSTRACT
Plasma total lipids, total cholesterol (cholesterol esters and free cholesterol) and oxysterol (mainly 7 beta-hydroxycholesterol (7 beta OH)) concentrations were significantly elevated in New Zealand rabbits fed a 2% cholesterol-containing diet with respect to controls fed the same diet without cholesterol. In addition, linoleic (182 n-6) and alpha-linolenic acid (183 n-3) plasma concentrations were significantly elevated in hypercholesterolemic rabbits, while concentrations of long-chain n-6 and n-3 derivatives were reduced. Studies in monocytic cell line THP-1 revealed that 7 beta OH markedly inhibited the conversion of 182 to 204 n-6 and of 183 to 226 n-3, indicating depression of the desaturation steps; in particular the inhibition was greater for the Delta 5 desaturation step. Furthermore, experiments of Real-Time PCR showed that 5-10 microM 7 beta OH decreased the Delta 5 gene expression. In conclusion, atherogenic oxysterols interfere with the production of long-chain polyunsaturated fatty acids from their precursors both in hypercholesterolemic rabbits and in cultured cells.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Ácidos Grasos Insaturados
/
Hidroxicolesteroles
/
Hipercolesterolemia
Límite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
Prostaglandins Leukot Essent Fatty Acids
Asunto de la revista:
ENDOCRINOLOGIA
Año:
2004
Tipo del documento:
Article
País de afiliación:
Italia