Atelocollagen-mediated synthetic small interfering RNA delivery for effective gene silencing in vitro and in vivo.
Nucleic Acids Res
; 32(13): e109, 2004 Jul 22.
Article
en En
| MEDLINE
| ID: mdl-15272050
ABSTRACT
Silencing gene expression by siRNAs is rapidly becoming a powerful tool for the genetic analysis of mammalian cells. However, the rapid degradation of siRNA and the limited duration of its action call for an efficient delivery technology. Accordingly, we describe here that Atelocollagen complexed with siRNA is resistant to nucleases and is efficiently transduced into cells, thereby allowing long-term gene silencing. Site-specific in vivo administration of an anti-luciferase siRNA/Atelocollagen complex reduced luciferase expression in a xenografted tumor. Furthermore, Atelocollagen-mediated transfer of siRNA in vivo showed efficient inhibition of tumor growth in an orthotopic xenograft model of a human non-seminomatous germ cell tumor. Thus, for clinical applications of siRNA, an Atelocollagen-based non-viral delivery method could be a reliable approach to achieve maximal function of siRNA in vivo.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Transducción Genética
/
Colágeno
/
ARN Interferente Pequeño
/
Interferencia de ARN
Tipo de estudio:
Evaluation_studies
Límite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
Nucleic Acids Res
Año:
2004
Tipo del documento:
Article
País de afiliación:
Japón