The patch-clamp and planar lipid bilayer techniques: powerful and versatile tools to investigate the CFTR Cl- channel.
J Cyst Fibros
; 3 Suppl 2: 101-8, 2004 Aug.
Article
en En
| MEDLINE
| ID: mdl-15463939
ABSTRACT
Using the patch-clamp (PC) and planar lipid bilayer (PLB) techniques the molecular behaviour of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel can be visualised in real-time. The PC technique is a highly powerful and versatile method to investigate CFTR's mechanism of action, interaction with other proteins and physiological role. Using the PLB technique, the structure and function of CFTR can be investigated free from the influence of other proteins. Here we discuss how these techniques are employed to investigate the CFTR Cl- channel with special emphasis on its permeation, conduction and gating properties.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Técnicas de Placa-Clamp
/
Regulador de Conductancia de Transmembrana de Fibrosis Quística
/
Membrana Dobles de Lípidos
Límite:
Humans
Idioma:
En
Revista:
J Cyst Fibros
Año:
2004
Tipo del documento:
Article
País de afiliación:
Reino Unido