Your browser doesn't support javascript.
loading
Low-density lipoprotein from apolipoprotein E-deficient mice induces macrophage lipid accumulation in a CD36 and scavenger receptor class A-dependent manner.
Zhao, Zhenze; de Beer, Maria C; Cai, Lei; Asmis, Reto; de Beer, Frederick C; de Villiers, Willem J S; van der Westhuyzen, Deneys R.
Afiliación
  • Zhao Z; Department of Internal Medicine and Graduate Center for Nutritional Sciences, University of Kentucky Medical Center, Lexington, KY 40536, USA.
Arterioscler Thromb Vasc Biol ; 25(1): 168-73, 2005 Jan.
Article en En | MEDLINE | ID: mdl-15514202
ABSTRACT

OBJECTIVE:

To investigate the potential of circulating low-density lipoprotein (LDL), isolated from apolipoprotein E (apoE)-deficient mice (E-/-LDL) and from LDL receptor-deficient mice (Lr-/-LDL), to induce foam cell formation. METHODS AND

RESULTS:

Binding studies using COS-7 cells overexpressing CD36, J774 cells, and mouse peritoneal macrophages (MPMs) unexpectedly showed for the first time that E-/-LDL, which is enriched in cholesterol, is a high-affinity ligand for CD36 and exhibited greater macrophage uptake than Lr-/-LDL or normal LDL. Minimal copper-mediated oxidization of Lr-/-LDL or C57LDL in vitro resulted in increased ligand internalization, although cell uptake of these oxidized LDLs was lower than that of E-/-LDL, even at oxidation levels similar to that found in E-/-LDL. Treatment of MPMs with E-/-LDL and Lr-/-LDL (to a 2- to 3-fold lesser extent), but not normal LDL, resulted in significant cellular cholesteryl ester accumulation and foam cell formation. Experiments using MPMs lacking CD36, scavenger receptor class A (SR-A), or both, indicated a major contribution of CD36 ( approximately 50%), and to a lesser extent, SR-A (24% to 30%), to E-/-LDL uptake.

CONCLUSIONS:

Because of its increased state of oxidation and high cholesterol content, LDL in apoE-deficient mice acts in a proatherogenic manner, without requiring further modification in the vascular wall, to induce foam cell formation through its uptake by scavenger receptors.
Asunto(s)
Buscar en Google
Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Apolipoproteínas E / Receptores Inmunológicos / Macrófagos Peritoneales / Antígenos CD36 / Metabolismo de los Lípidos Límite: Animals Idioma: En Revista: Arterioscler Thromb Vasc Biol Asunto de la revista: ANGIOLOGIA Año: 2005 Tipo del documento: Article País de afiliación: Estados Unidos
Buscar en Google
Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Apolipoproteínas E / Receptores Inmunológicos / Macrófagos Peritoneales / Antígenos CD36 / Metabolismo de los Lípidos Límite: Animals Idioma: En Revista: Arterioscler Thromb Vasc Biol Asunto de la revista: ANGIOLOGIA Año: 2005 Tipo del documento: Article País de afiliación: Estados Unidos