Your browser doesn't support javascript.
loading
Polo-box motif targets a centrosome regulator, RanGTPase.
Jang, Young-Joo; Ji, Jae-Hoon; Ahn, Ji-Hee; Hoe, Kwang-Lae; Won, Misun; Im, Dong-Soo; Chae, Suhn-Kee; Song, Sukgil; Yoo, Hyang-Sook.
Afiliación
  • Jang YJ; Genome Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 52 Oeun-Dong, Yusong-Gu, Daejeon 305-333, Republic of Korea. youngjoo@kribb.re.kr
Biochem Biophys Res Commun ; 325(1): 257-64, 2004 Dec 03.
Article en En | MEDLINE | ID: mdl-15522227
ABSTRACT
Mammalian polo-like kinase (Plk) acts at various stages in early and late mitosis. Plk1 localizes in the centrosome, the central spindle, the midbody as well as the kinetochore. The non-catalytic region in the C-terminus of Plk1 has conserved sequence motifs, named polo-boxes. These motifs are important for Plk localization. GFP protein fused with the core sequences of polo-box (50 amino acids) localized Plk to target organelles. We screened for Plk interacting proteins by constructing a tandem repeat of the polo-box motif, and used it as bait in the two-hybrid system with HeLa cell cDNA library. RanGTPase was detected as a positive clone. Through in vitro and in vivo protein binding analysis in synchronized cells by thymidine block and by nocodazole treatment, we confirmed the interaction between endogenous Ran and Plk1. We showed that endogenous Ran and Plk1 proteins were co-localized to centrosomes, which is a major target organelle of endogenous Plk1, in early mitotic cells by immunofluorescence. Finally, we demonstrated that Plk1 phosphorylated RanBPM, a Ran-binding protein in microtubule organizing center, through the interaction with Ran. These data suggested that the core motif of polo-box is sufficient for Plk1-targeting, and that Plk1 may play roles in centrosome through recruitment and/or activation of Ran/RanBPM proteins.
Asunto(s)
Buscar en Google
Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas Quinasas / Centrosoma / Proteína de Unión al GTP ran / Secuencias de Aminoácidos Límite: Animals / Humans Idioma: En Revista: Biochem Biophys Res Commun Año: 2004 Tipo del documento: Article
Buscar en Google
Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas Quinasas / Centrosoma / Proteína de Unión al GTP ran / Secuencias de Aminoácidos Límite: Animals / Humans Idioma: En Revista: Biochem Biophys Res Commun Año: 2004 Tipo del documento: Article