Electrospray tandem mass spectrometry for structural characterization of aporphine- benzylisoquinoline alkaloids.
Eur J Mass Spectrom (Chichester)
; 10(5): 683-9, 2004.
Article
en En
| MEDLINE
| ID: mdl-15531802
ABSTRACT
Electrospray mass spectrometry and tandem mass spectrometry techniques were utilized to elucidate the structures of ten aporphine-benzylisoquinoline alkaloids, consisting of monoether link between aporphine and benzyltetrahydroisoquinoline units, which were isolated and identified previously from a variety of Thalictrum sp. (Ranunculaceae family) based mainly on the UV, IR, CD, NMR, EI-MS, CI-MS, derivatization, and chemical degradation techniques. In this investigation, protonated molecules, [M+H]+ ions, for nine tertiary alkaloids, a molecular ion, [M+'] ion, for a quaternary alkaloid, and very intense doubly- protonated molecules, [M+2H]2+ ions (100% of relative abundance) in Q1 Scan MS spectra, and prominent as well as diagnostic product ions for structural information in the tandem MS/MS spectra were observed for all investigated alkaloids each in nanogram quantities. More than 10 microg quantities of each investigated alkaloid or other isoquinoline and aporphine analogs needed for the CI-MS, EI-MS and FAB-MS analysis from the previous studies.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Aporfinas
/
Protones
/
Espectrometría de Masa por Ionización de Electrospray
/
Thalictrum
/
Bencilisoquinolinas
Idioma:
En
Revista:
Eur J Mass Spectrom (Chichester)
Año:
2004
Tipo del documento:
Article
País de afiliación:
Estados Unidos