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A case study of de novo sequence analysis of N-sulfonated peptides by MALDI TOF/TOF mass spectrometry.
Samyn, Bart; Debyser, Griet; Sergeant, Kjell; Devreese, Bart; Van Beeumen, Jozef.
Afiliación
  • Samyn B; Department of Biochemistry, Physiology, and Microbiology, Laboratory of Protein Biochemistry and Protein Engineering, University of Gent, K.L Ledeganckstraat 35, B-9000 Gent, Belgium.
J Am Soc Mass Spectrom ; 15(12): 1838-52, 2004 Dec.
Article en En | MEDLINE | ID: mdl-15589760
The simplicity and sensitivity of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry have increased its application in recent years. The most common method of "peptide mass fingerprint" analysis often does not provide robust identification. Additional sequence information, obtained by post-source decay or collision induced dissociation, provides additional constraints for database searches. However, de novo sequencing by mass spectrometry is not yet common practice, most likely because of the difficulties associated with the interpretation of high and low energy CID spectra. Success with this type of sequencing requires full sequence coverage and demands better quality spectra than those typically used for data base searching. In this report we show that full-length de novo sequencing is possible using MALDI TOF/TOF analysis. The interpretation of MS/MS data is facilitated by N-terminal sulfonation after protection of lysine side chains (Keough et al., Proc. Natl. Acad. Sci. U.S.A. 1999, 96, 7131-7136). Reliable de novo sequence analysis has been obtained using sub-picomol quantities of peptides and peptide sequences of up to 16 amino acid residues in length have been determined. The simple, predictable fragmentation pattern allows routine de novo interpretation, either manually or using software. Characterization of the complete primary structure of a peptide is often hindered because of differences in fragmentation efficiencies and in specific fragmentation patterns for different peptides. These differences are controlled by various structural parameters including the nature of the residues present. The influence of the presence of internal Pro, acidic and basic residues on the TOF/TOF fragmentation pattern will be discussed, both for underivatized and guanidinated/sulfonated peptides.
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Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Mapeo Peptídico / Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción / Espectrometría de Masa por Ionización de Electrospray Límite: Animals Idioma: En Revista: J Am Soc Mass Spectrom Año: 2004 Tipo del documento: Article País de afiliación: Bélgica
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Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Mapeo Peptídico / Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción / Espectrometría de Masa por Ionización de Electrospray Límite: Animals Idioma: En Revista: J Am Soc Mass Spectrom Año: 2004 Tipo del documento: Article País de afiliación: Bélgica