In vivo labeling of neuroblasts in the subventricular zone of rats.
J Neurosci Methods
; 142(2): 285-93, 2005 Mar 30.
Article
en En
| MEDLINE
| ID: mdl-15698668
ABSTRACT
The studies of neural stem cell fate, as well as the possibility to genetically manipulate them, represent important tools for modern neuroscience research. Furthermore, the potential use of these cells in treatment of neurological disorders makes these methods valuable for the development of new treatment paradigms. Here we report a method to genetically mark and modify neuroblasts and progenitor cells in the subventricular zone of post-natal rats using retroviral vectors. Using GFP as a marker gene we were able to follow the cells as they migrate and differentiate into olfactory interneurons. The cells were found in the olfactory bulb already 1 week after injection of the vector and after 3 weeks all cells had reached this area. There was a higher efficiency of the labeling of cells in neonatal rats compared to adults but injecting directly into the subventricular zone could to some extent counteract this effect. However, the cell types generated by the GFP positive cells were the same in neonatal and adult animals. This method will be a powerful tool to study the genetic interplay involved in neural stem cell differentiation and may be instrumental in finding a way to instruct these cells to participate in brain repair in the adult central nervous system.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Coloración y Etiquetado
/
Células Madre
/
Ventrículos Laterales
/
Neuronas
Límite:
Animals
Idioma:
En
Revista:
J Neurosci Methods
Año:
2005
Tipo del documento:
Article
País de afiliación:
Suecia