ATP potentiates the formation of AChR aggregate in the co-culture of NG108-15 cells with C2C12 myotubes.
FEBS Lett
; 579(11): 2469-74, 2005 Apr 25.
Article
en En
| MEDLINE
| ID: mdl-15848190
ABSTRACT
The role of adenosine 5'-triphosphate (ATP) and P2Y(1) nucleotide receptor in potentiating agrin-induced acetylcholine receptor (AChR) aggregation is being demonstrated in a co-culture system of NG108-15 cell, a mouse neuroblastoma X rat glioma hybrid cell line that resembles spinal motor neuron, with C2C12 myotube. In the co-cultures, antagonized P2Y(1) receptors showed a reduction in NG108-15 cell-induced AChR aggregation. Parallel to this observation, cultured NG108-15 cell secreted ATP into the conditioned medium in a time-dependent manner. Enhancement of ATP release from the cultured NG108-15 cells by overexpression of active mutants of small GTPases increased the aggregation of AChRs in co-culturing with C2C12 myotubes. In addition, ecto-nucleotidase was revealed in the co-culture, which rapidly degraded the applied ATP. These results support the notion that ATP has a role in directing the formation of post-synaptic apparatus in vertebrate neuromuscular junctions.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Adenosina Trifosfato
/
Receptores Colinérgicos
/
Fibras Musculares Esqueléticas
Límite:
Animals
Idioma:
En
Revista:
FEBS Lett
Año:
2005
Tipo del documento:
Article
País de afiliación:
China