Anaerobic induction of isocitrate lyase and malate synthase in submerged rice seedlings indicates the important metabolic role of the glyoxylate cycle.
Acta Biochim Biophys Sin (Shanghai)
; 37(6): 406-14, 2005 Jun.
Article
en En
| MEDLINE
| ID: mdl-15944756
ABSTRACT
The glyoxylate cycle is a modified form of the tricarboxylic acid cycle that converts C2 compounds into C4 dicarboxylic acids at plant developmental stages. By studying submerged rice seedlings, we revealed the activation of the glyoxylate cycle by identifying the increased transcripts of mRNAs of the genes of isocitrate lyase (ICL) and malate synthase (MS), two characteristic enzymes of the glyoxylate cycle. Northern blot analysis showed that ICL and MS were activated in the prolonged anaerobic environment. The activity assay of pyruvate decarboxylase and ICL in the submerged seedlings indicated an 8.8-fold and 3.5-fold increase over that in the unsubmerged seedlings, respectively. The activity assay of acetyl-coenzyme A synthetase in the submerged seedlings indicated a 3-fold increase over that in the unsubmerged seedlings, which is important for initiating acetate metabolism. Consequently, we concluded that the glyoxylate cycle was involved in acetate metabolism under anaerobic conditions.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Oryza
/
Inducción Enzimática
/
Ciclo del Ácido Cítrico
/
Plantones
/
Glioxilatos
/
Anaerobiosis
/
Isocitratoliasa
/
Malato Sintasa
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Acta Biochim Biophys Sin (Shanghai)
Asunto de la revista:
BIOFISICA
/
BIOQUIMICA
Año:
2005
Tipo del documento:
Article
País de afiliación:
China